› 2yeu

X-ray diffraction
2Å resolution

Structural and functional insights of DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans, complex with Gd

Released:
DOI: 10.2210/pdb2yeu/pdb
PDB entry 2yeu coloured by chain, front view.
PDB entry 2yeu coloured by chain, side view.
PDB entry 2yeu coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans.
Gonçalves AMD, de Sanctis D, McSweeney SM
J Biol Chem 286 30691-30705 (2011)
PMID: 21733847

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HAD superfamily Cof-like phosphohydrolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 154 amino acids
Theoretical weight: 16.71 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RS96 (Residues: 1-148; Coverage: 100%)
Gene name: DR_2231
Sequence domains: Phosphoribosyl-ATP pyrophosphohydrolase
Structure domains: putative ntp pyrophosphohydrolase like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 16 x SO4
Ligand GOL 21 x GOL
Ligand GD 6 x GD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 67.82Å b: 110.75Å c: 165.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Preventive DNA repair by sanitizing the cellular (deoxy)nucleoside triphosphate pool.
Nagy et al. (2014)
1 more

1 mention without citation

Structural and functional insights into DR2231 protein, the MazG-like nucleoside triphosphate pyrophosphohydrolase from Deinococcus radiodurans.
Gonçalves et al. (2011)