› 2yez

X-ray diffraction
2.9Å resolution

COMPLEX OF A B21 CHICKEN MHC CLASS I MOLECULE AND A 10MER CHICKEN PEPTIDE

Released:
DOI: 10.2210/pdb2yez/pdb
PDB entry 2yez coloured by chain, front view.
PDB entry 2yez coloured by chain, side view.
PDB entry 2yez coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding.
Chappell P, Meziane el K, Harrison M, Magiera Ł, Hermann C, Mears L, Wrobel AG, Durant C, Nielsen LL, Buus S, Ternette N, Mwangi W, Butter C, Nair V, Ahyee T, Duggleby R, Madrigal A, Roversi P, Lea SM, Kaufman J
Elife 4 e05345 (2015)
PMID: 25860507

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-149331 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 36.86 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95601 (Residues: 1-291; Coverage: 81%)
Gene names: B-FIV, BF2, BFIV21
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 11.06 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21611 (Residues: 22-119; Coverage: 100%)
Gene name: B2M
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
14-3-3 protein theta Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: Q5ZMD1 (Residues: 110-119; Coverage: 4%)
Gene names: RCJMB04_2i3, YWHAQ

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 60.57Å b: 68.98Å c: 94.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.251 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

A cold-blooded view of adaptive immunity.
Flajnik MF. (2018)
18 more

1 mention without citation

Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding.
Chappell et al. (2015)