› 2yfp

X-ray diffraction
2.6Å resolution

STRUCTURE OF YELLOW-EMISSION VARIANT OF GFP

Released:
DOI: 10.2210/pdb2yfp/pdb
PDB entry 2yfp coloured by chain, front view.
PDB entry 2yfp coloured by chain, side view.
PDB entry 2yfp coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein.
Wachter RM, Elsliger MA, Kallio K, Hanson GT, Remington SJ
Structure 6 1267-77 (1998)
PMID: 9782051

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.88 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.994Å b: 62.756Å c: 69.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 not available
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
34 more

3 mentions without citation

Members of a novel protein family containing microneme adhesive repeat domains act as sialic acid-binding lectins during host cell invasion by apicomplexan parasites.
Friedrich et al. (2010)
2 more