› 2yht

X-ray diffraction
2.9Å resolution

Crystal structure of Hfq riboregulator from E. coli (P1 space group)

Released:
DOI: 10.2210/pdb2yht/pdb
PDB entry 2yht coloured by chain, front view.
PDB entry 2yht coloured by chain, side view.
PDB entry 2yht coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Exploiting Protein Engineering and Crystal Polymorphism for Successful X-Ray Structure Determination
Bonnefond L, Schellenberger P, Basquin J, Demangeat G, Ritzenthaler C, Chenevert R, Balg C, Frugier M, Rudinger-Thirion J, Giege R, Lorber B, Sauter C
Cryst Growth Des 11 4334- (2011)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 74 amino acids
Theoretical weight: 8.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6X3 (Residues: 1-72; Coverage: 71%)
Gene names: JW4130, b4172, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 61.2Å b: 61.2Å c: 53.1Å
α: 82.6° β: 87.3° γ: 60°
R-values:
R R work R free
0.183 0.181 0.228
Expression system: Escherichia coli BL21(DE3)

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