› 2yjh

X-ray diffraction
2.55Å resolution

Thiol Peroxidase from Yersinia Psuedotuberculosis, inactive mutant C61S

Released:
DOI: 10.2210/pdb2yjh/pdb
PDB entry 2yjh coloured by chain, front view.
PDB entry 2yjh coloured by chain, side view.
PDB entry 2yjh coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis
Primary publication:
Structural characterisation of Tpx from Yersinia pseudotuberculosis reveals insights into the binding of salicylidene acylhydrazide compounds.
Gabrielsen M, Beckham KS, Feher VA, Zetterström CE, Wang D, Müller S, Elofsson M, Amaro RE, Byron O, Roe AJ
PLoS One 7 e32217 (2012)
PMID: 22384182

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol peroxidase Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 21.38 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q66A71 (Residues: 1-167; Coverage: 100%)
Gene names: YPTB2261, tpx
Sequence domains: Redoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P64
Unit cell:
a: 65.03Å b: 65.03Å c: 85.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.313 0.312 0.337
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Chemical inhibitors of the type three secretion system: disarming bacterial pathogens.
Duncan et al. (2012)
4 more

3 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
2 more