› 2yjv

X-ray diffraction
2.8Å resolution

Crystal structure of E. coli regulator of ribonuclease activity A (RraA) bound to fragment of DEAD-box protein RhlB

Released:
DOI: 10.2210/pdb2yjv/pdb
PDB entry 2yjv coloured by chain, front view.
PDB entry 2yjv coloured by chain, side view.
PDB entry 2yjv coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.
Pietras Z, Hardwick SW, Swiezewski S, Luisi BF
J Biol Chem 288 31919-29 (2013)
PMID: 24045937

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-141735 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of ribonuclease activity A Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 161 amino acids
Theoretical weight: 17.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A8R0 (Residues: 1-161; Coverage: 100%)
Gene names: JW3900, b3929, menG, rraA, yiiV
Sequence domains: Aldolase/RraA
Structure domains: Ribonuclease E inhibitor RraA/RraA-like
ATP-DEPENDENT RNA HELICASE RHLB Chains: M, N
Molecule details ›
Chains: M, N
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 259.63Å b: 69.07Å c: 123.3Å
α: 90° β: 109.17° γ: 90°
R-values:
R R work R free
0.224 0.221 0.288
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Bacterial versatility requires DEAD-box RNA helicases.
Redder et al. (2015)
3 more

2 mentions without citation

Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.
Pietras et al. (2013)
1 more