› 2ylc

X-ray diffraction
1.3Å resolution

Structure of Salmonella typhimurium Hfq in complex with U6 RNA

Released:
DOI: 10.2210/pdb2ylc/pdb
PDB entry 2ylc coloured by chain, front view.
PDB entry 2ylc coloured by chain, side view.
PDB entry 2ylc coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structural basis for RNA 3'-end recognition by Hfq.
Sauer E, Weichenrieder O
Proc Natl Acad Sci U S A 108 13065-70 (2011)
PMID: 21737752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.26 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1R0 (Residues: 1-72; Coverage: 71%)
Gene names: STM4361, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

2 bound ligands:
Ligand SCN 2 x SCN
Ligand U 1 x U
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6
Unit cell:
a: 61.19Å b: 61.19Å c: 28.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.134 0.132 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

Regulation by small RNAs in bacteria: expanding frontiers.
Storz et al. (2011)
34 more

8 mentions without citation

Structure and RNA-binding properties of the bacterial LSm protein Hfq.
Sauer E. (2013)
7 more