› 2yll

X-ray diffraction
1.85Å resolution

Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis

Released:
DOI: 10.2210/pdb2yll/pdb
Model geometry
Fit model/data
PDB entry 2yll coloured by chain, front view.
PDB entry 2yll coloured by chain, side view.
PDB entry 2yll coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Inhibition of the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis.
Pluvinage B, Higgins MA, Abbott DW, Robb C, Dalia AB, Deng L, Weiser JN, Parsons TB, Fairbanks AJ, Vocadlo DJ, Boraston AB
Structure 19 1603-14 (2011)
PMID: 22078560

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-N-acetylhexosaminidase Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 50.36 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli
UniProt:
  • Canonical: P49610 (Residues: 181-614; Coverage: 34%)
Gene names: SP_0057, strH
Sequence domains: Glycosyl hydrolase family 20, catalytic domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand PE4 1 x PE4
Ligand EDO 2 x EDO
Ligand IOD 67 x IOD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: I222
Unit cell:
a: 78.52Å b: 109.42Å c: 112.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.2
Expression system: Escherichia coli

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Citations

6 review citations

The chitinolytic machinery of Serratia marcescens--a model system for enzymatic degradation of recalcitrant polysaccharides.
Vaaje-Kolstad et al. (2013)
5 more