› 2ynj

Electron Microscopy
8.4Å resolution

GroEL at sub-nanometer resolution by Constrained Single Particle Tomography

Released:
DOI: 10.2210/pdb2ynj/pdb
PDB entry 2ynj coloured by chain, front view.
PDB entry 2ynj coloured by chain, side view.
PDB entry 2ynj coloured by chain, top view.
Source organism: Escherichia coli UTI89
Primary publication:
Protein secondary structure determination by constrained single-particle cryo-electron tomography.
Bartesaghi A, Lecumberry F, Sapiro G, Subramaniam S
Structure 20 2003-13 (2012)
PMID: 23217682
Related structures: EMD-2221

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 524 amino acids
Theoretical weight: 55.22 KDa
Source organism: Escherichia coli UTI89
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1R3B6 (Residues: 2-525; Coverage: 96%)
Gene names: UTI89_C4741, groEL, groL
Sequence domains: TCP-1/cpn60 chaperonin family

Ligands and Environments

3 bound ligands:
Ligand AGS 14 x AGS
Ligand TL 56 x TL
Ligand MG 14 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.4Å
Relevant EMDB volumes: EMD-2221
Expression system: Escherichia coli

Quick links

Citations

10 review citations

HIV-1 envelope glycoprotein structure.
Merk et al. (2013)
9 more

5 mentions without citation

Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification.
Ravelli et al. (2020)
4 more