› 2ynz

X-ray diffraction
1.4Å resolution

Salmonella enterica SadA 823-947 fused to a GCN4 adaptor (SadAK5)

Released:
DOI: 10.2210/pdb2ynz/pdb
PDB entry 2ynz coloured by chain, front view.
PDB entry 2ynz coloured by chain, side view.
PDB entry 2ynz coloured by chain, top view.
Source organisms:
Primary publication:
Complete fiber structures of complex trimeric autotransporter adhesins conserved in enterobacteria.
Hartmann MD, Grin I, Dunin-Horkawicz S, Deiss S, Linke D, Lupas AN, Hernandez Alvarez B
Proc Natl Acad Sci U S A 109 20907-12 (2012)
PMID: 23213248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autotransporter adhesin SadA; General control transcription factor GCN4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 154 amino acids
Theoretical weight: 16.68 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P03069 (Residues: 250-278; Coverage: 10%)
  • Canonical: Q8ZL64 (Residues: 823-947; Coverage: 9%)
Gene names: AAS101, AAS3, ARG9, GCN4, STM3691, YEL009C, sadA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NO3 4 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 44.69Å b: 60.3Å c: 135.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.232
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective.
Chagnot et al. (2013)
8 more

4 mentions without citation

Recent advances in the understanding of trimeric autotransporter adhesins.
Kiessling et al. (2020)
3 more