› 2yo0

X-ray diffraction
2.8Å resolution

Salmonella enterica SadA 1049-1304 fused to GCN4 adaptors (SadAK9-cfI)

Released:
DOI: 10.2210/pdb2yo0/pdb
PDB entry 2yo0 coloured by chain, front view.
PDB entry 2yo0 coloured by chain, side view.
PDB entry 2yo0 coloured by chain, top view.
Source organisms:
Primary publication:
Complete fiber structures of complex trimeric autotransporter adhesins conserved in enterobacteria.
Hartmann MD, Grin I, Dunin-Horkawicz S, Deiss S, Linke D, Lupas AN, Hernandez Alvarez B
Proc Natl Acad Sci U S A 109 20907-12 (2012)
PMID: 23213248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autotransporter adhesin SadA; General control transcription factor GCN4 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 34.01 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZL64 (Residues: 1038-1304; Coverage: 19%)
  • Canonical: P03069 (Residues: 250-278; Coverage: 10%)
Gene names: AAS101, AAS3, ARG9, GCN4, STM3691, YEL009C, sadA
Sequence domains:
Structure domains: Serralysin-like metalloprotease, C-terminal

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P63
Unit cell:
a: 54Å b: 54Å c: 306.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.221 0.297
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Proteinaceous determinants of surface colonization in bacteria: bacterial adhesion and biofilm formation from a protein secretion perspective.
Chagnot et al. (2013)
9 more

3 mentions without citation

Complete fiber structures of complex trimeric autotransporter adhesins conserved in enterobacteria.
Hartmann et al. (2012)
2 more