› 2ypf

X-ray diffraction
2.55Å resolution

Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism

Released:
DOI: 10.2210/pdb2ypf/pdb
PDB entry 2ypf coloured by chain, front view.
PDB entry 2ypf coloured by chain, side view.
PDB entry 2ypf coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism.
Stella S, Molina R, Yefimenko I, Prieto J, Silva G, Bertonati C, Juillerat A, Duchateau P, Montoya G
Acta Crystallogr D Biol Crystallogr 69 1707-16 (2013)
PMID: 23999294

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115953 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Avirulence protein AvrBs3 Chain: A
Molecule details ›
Chain: A
Length: 758 amino acids
Theoretical weight: 78.65 KDa
Source organism: Xanthomonas campestris
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q3ZD73 (Residues: 153-905; Coverage: 56%)
Gene name: hax2
Sequence domains: TAL effector repeat
5'-D(*TP*TP*TP*AP*TP*AP*TP*AP*AP*AP*CP*CP*TP*AP *AP*CP*CP*CP*TP*CP*TP*AP)-3' Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.63 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*TP*AP*GP*AP*GP*GP*GP*TP*TP*AP*GP*GP*TP*TP *TP*AP*TP*AP*TP*AP*AP)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.56 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 151.11Å b: 100.25Å c: 61.37Å
α: 90° β: 102.55° γ: 90°
R-values:
R R work R free
0.239 0.236 0.29
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

3 review citations

The genome editing revolution: A CRISPR-Cas TALE off-target story.
Stella et al. (2016)
2 more

4 mentions without citation

α-Helices in the Type III Secretion Effectors: A Prevalent Feature with Versatile Roles.
Gazi et al. (2021)
3 more