› 2ypo

X-ray diffraction
2Å resolution

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase with phenylalanine bound in only one site

Released:
DOI: 10.2210/pdb2ypo/pdb
PDB entry 2ypo coloured by chain, front view.
PDB entry 2ypo coloured by chain, side view.
PDB entry 2ypo coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Three sites and you are out: ternary synergistic allostery controls aromatic amino acid biosynthesis in Mycobacterium tuberculosis.
Blackmore NJ, Reichau S, Jiao W, Hutton RD, Baker EN, Jameson GB, Parker EJ
J Mol Biol 425 1582-92 (2013)
PMID: 23274137

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase AroG Chains: A, B
Molecule details ›
Chains: A, B
Length: 462 amino acids
Theoretical weight: 50.7 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: O53512 (Residues: 1-462; Coverage: 100%)
Gene names: Rv2178c, aroG
Sequence domains: Class-II DAHP synthetase family

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand SO4 5 x SO4
Ligand GOL 6 x GOL
Ligand PHE 2 x PHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3221
Unit cell:
a: 203.969Å b: 203.969Å c: 66.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.139 0.138 0.159
Expression system: Escherichia coli

Quick links

Citations

8 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
7 more

1 mention without citation

Probing the Sophisticated Synergistic Allosteric Regulation of Aromatic Amino Acid Biosynthesis in Mycobacterium tuberculosis Using ᴅ-Amino Acids.
Reichau et al. (2016)