› 2yq5

X-ray diffraction
2.75Å resolution

Crystal Structure of D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus: NAD complexed form

Released:
DOI: 10.2210/pdb2yq5/pdb
PDB entry 2yq5 coloured by chain, front view.
PDB entry 2yq5 coloured by chain, side view.
PDB entry 2yq5 coloured by chain, top view.
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Primary publication:
Structural characterization of a D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus.
Holton SJ, Anandhakrishnan M, Geerlof A, Wilmanns M
J Struct Biol 181 179-84 (2013)
PMID: 23110853

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-isomer specific 2-hydroxyacid dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 343 amino acids
Theoretical weight: 38.03 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1GAA2 (Residues: 1-333; Coverage: 100%)
Gene name: Ldb1010
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NAD 4 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 92.718Å b: 110.012Å c: 147.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Molecular mechanisms of vancomycin resistance.
Stogios et al. (2020)
8 other articles