› 2yrf

X-ray diffraction
2.7Å resolution

Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion

Released:
DOI: 10.2210/pdb2yrf/pdb
PDB entry 2yrf coloured by chain, front view.
PDB entry 2yrf coloured by chain, side view.
PDB entry 2yrf coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.
Tamura H, Saito Y, Ashida H, Inoue T, Kai Y, Yokota A, Matsumura H
Protein Sci 17 126-35 (2008)
PMID: 18156470

Function and Biology Details

Reaction catalysed: 
S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylthioribose-1-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 41.21 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31662 (Residues: 1-353; Coverage: 100%)
Gene names: BSU13550, mtnA, ykrS
Sequence domains: Initiation factor 2 subunit family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P41
Unit cell:
a: 69.273Å b: 69.273Å c: 154.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.284
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Metabolic characteristics and importance of the universal methionine salvage pathway recycling methionine from 5'-methylthioadenosine.
Albers E. (2009)
1 more

2 mentions without citation

Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.
Tamura et al. (2008)
1 more