2yvs

X-ray diffraction
2Å resolution

Crystal structure of glycolate oxidase subunit GlcE from Thermus thermophilus HB8

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Yoneda K, Sakuraba H, Kuramitsu S, Ohshima T

Function and Biology Details

Reaction catalysed:
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-178253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.2 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SM78 (Residues: 1-219; Coverage: 100%)
Gene name: TTHA0065
Sequence domains: Family of unknown function (DUF5639)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 69.88Å b: 71.69Å c: 89.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.249
Expression system: Escherichia coli