› 2ywg

X-ray diffraction
2.94Å resolution

Crystal structure of GTP-bound LepA from Aquifex aeolicus

Released:
DOI: 10.2210/pdb2ywg/pdb
PDB entry 2ywg coloured by chain, front view.
PDB entry 2ywg coloured by chain, side view.
PDB entry 2ywg coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Entry authors: Kawazoe M, Takemoto C, Kaminishi T, Nishino A, Nakayama-Ushikoshi R, Hanawa-Suetsugu K, Terada T, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor 4 Chain: A
Molecule details ›
Chain: A
Length: 600 amino acids
Theoretical weight: 68.36 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67618 (Residues: 1-600; Coverage: 100%)
Gene names: aq_1725, lepA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GTP 1 x GTP
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 81.518Å b: 116.348Å c: 122.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.265
Expression system: Escherichia coli

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