› 2ywv

X-ray diffraction
1.75Å resolution

Crystal structure of SAICAR synthetase from Geobacillus kaustophilus

Released:
DOI: 10.2210/pdb2ywv/pdb
PDB entry 2ywv coloured by chain, front view.
PDB entry 2ywv coloured by chain, side view.
PDB entry 2ywv coloured by chain, top view.
Source organism: Geobacillus kaustophilus
Entry authors: Kanagawa M, Baba S, Kuramitsu S, Yokoyama S, Kawai G, Sampei G, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 244 amino acids
Theoretical weight: 27.33 KDa
Source organism: Geobacillus kaustophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5L3D5 (Residues: 1-242; Coverage: 100%)
Gene names: GK0260, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 7 x SO4
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P1
Unit cell:
a: 49.89Å b: 56.397Å c: 56.41Å
α: 76.39° β: 64.36° γ: 64.35°
R-values:
R R work R free
0.188 0.188 0.191
Expression system: Escherichia coli

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