› 2yx5

X-ray diffraction
2.3Å resolution

Crystal Structure of Methanocaldococcus jannaschii PurS, One of the Subunits of Formylglycinamide Ribonucleotide Amidotransferase in the Purine Biosynthetic Pathway

Released:
DOI: 10.2210/pdb2yx5/pdb
PDB entry 2yx5 coloured by chain, front view.
PDB entry 2yx5 coloured by chain, side view.
PDB entry 2yx5 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Kanagawa M, Baba S, Agari Y, Chen LQ, Fu Z-Q, Chrzas J, Wang BC, Kuramitsu S, Yokoyama S, Kawai G, Sampei G, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylformylglycinamidine synthase subunit PurS Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.71 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58988 (Residues: 1-83; Coverage: 100%)
Gene names: MJ1593, purS
Sequence domains: Phosphoribosylformylglycinamidine (FGAM) synthase
Structure domains: Phosphoribosylformylglycinamidine synthase subunit PurS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6422
Unit cell:
a: 96.251Å b: 96.251Å c: 48.257Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.24 0.284
Expression system: Escherichia coli

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