› 2yxe

X-ray diffraction
2Å resolution

Crystal structure of L-isoaspartyl protein carboxyl methyltranferase

Released:
DOI: 10.2210/pdb2yxe/pdb
PDB entry 2yxe coloured by chain, front view.
PDB entry 2yxe coloured by chain, side view.
PDB entry 2yxe coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Entry authors: Padmanabhan B, Bessho Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-L-isoaspartate O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 23.79 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57636 (Residues: 1-215; Coverage: 100%)
Gene names: MJ0172, pcm
Sequence domains: Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 60.267Å b: 84.448Å c: 123.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.231
Expression system: Escherichia coli

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