› 2yxl

X-ray diffraction
2.55Å resolution

Crystal Structure of PH0851

Released:
DOI: 10.2210/pdb2yxl/pdb
PDB entry 2yxl coloured by chain, front view.
PDB entry 2yxl coloured by chain, side view.
PDB entry 2yxl coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structure of an archaeal homologue of the bacterial Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase.
Hikida Y, Kuratani M, Bessho Y, Sekine SI, Yokoyama S
Acta Crystallogr D Biol Crystallogr 66 1301-7 (2010)
PMID: 21123870

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAM-dependent MTase RsmB/NOP-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 51.72 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58581 (Residues: 1-450; Coverage: 100%)
Gene name: PH0851
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SFG 1 x SFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3221
Unit cell:
a: 116.621Å b: 116.621Å c: 93.684Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.246
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Cryo-EM study of an archaeal 30S initiation complex gives insights into evolution of translation initiation.
Coureux et al. (2020)
2 more

3 mentions without citation

Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.
Liu et al. (2017)
2 more