› 2yxo

X-ray diffraction
1.6Å resolution

Histidinol Phosphate Phosphatase complexed with Sulfate

Released:
DOI: 10.2210/pdb2yxo/pdb
PDB entry 2yxo coloured by chain, front view.
PDB entry 2yxo coloured by chain, side view.
PDB entry 2yxo coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8.
Omi R, Goto M, Miyahara I, Manzoku M, Ebihara A, Hirotsu K
Biochemistry 46 12618-27 (2007)
PMID: 17929834

Function and Biology Details

Reaction catalysed: 
L-histidinol phosphate + H(2)O = L-histidinol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidinol-phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.02 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLG2 (Residues: 1-267; Coverage: 100%)
Gene name: TTHA0331
Sequence domains:
Structure domains: Metal-dependent hydrolases

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand ZN 2 x ZN
Ligand FE 4 x FE
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 84.771Å b: 97.19Å c: 74.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biochemical functional predictions for protein structures of unknown or uncertain function.
Mills et al. (2015)
17 other articles

2 mentions without citation

Prediction of ligand binding sites using homologous structures and conservation at CASP8.
Wass et al. (2009)
1 more