2yxp

X-ray diffraction
1.53Å resolution

The Effect of Deuteration on Protein Structure A High Resolution Comparison of Hydrogenous and Perdeuterated Haloalkane Dehalogenase

Released:

Function and Biology Details

Reaction catalysed:
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-149750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: X
Molecule details ›
Chain: X
Length: 310 amino acids
Theoretical weight: 35.18 KDa
Source organism: Xanthobacter autotrophicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22643 (Residues: 1-310; Coverage: 100%)
Gene name: dhlA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 92.666Å b: 72.184Å c: 41.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.185
Expression system: Escherichia coli BL21(DE3)