› 2yxq

X-ray diffraction
3.5Å resolution

The plug domain of the SecY protein stablizes the closed state of the translocation channel and maintains a membrane seal

Released:
DOI: 10.2210/pdb2yxq/pdb
PDB entry 2yxq coloured by chain, front view.
PDB entry 2yxq coloured by chain, side view.
PDB entry 2yxq coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Puzzle plugged by protein pore plasticity.
Minor DL
Mol Cell 26 459-60 (2007)
PMID: 17531803

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158008 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein translocase subunit SecY Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 46.94 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60175 (Residues: 1-436; Coverage: 99%)
Gene names: MJ0478, secY
Sequence domains:
Structure domains: SecY subunit domain
Protein translocase subunit SecE Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.45 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57817 (Residues: 1-74; Coverage: 100%)
Gene names: MJ0371, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Structure domains: Preprotein translocase SecE subunit
Preprotein translocase subunit SecG Chain: C
Molecule details ›
Chain: C
Length: 53 amino acids
Theoretical weight: 5.97 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: P60460 (Residues: 1-53; Coverage: 100%)
Gene names: MJ0912.1, secG
Sequence domains: Sec61beta family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 92.235Å b: 148.489Å c: 81.072Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.301 0.301 0.317
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From the Sec complex to the membrane insertase YidC.
Kuhn A. (2009)

1 mention without citation

The roles of pore ring and plug in the SecY protein-conducting channel.
Gumbart et al. (2008)