› 2yy5

X-ray diffraction
2.55Å resolution

Crystal Structure of tryptophanyl-tRNA synthetase from Mycoplasma pneumoniae

Released:
DOI: 10.2210/pdb2yy5/pdb
PDB entry 2yy5 coloured by chain, front view.
PDB entry 2yy5 coloured by chain, side view.
PDB entry 2yy5 coloured by chain, top view.
Source organism: Mycoplasma pneumoniae M129
Entry authors: Ishii T, Bessho Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 348 amino acids
Theoretical weight: 39.31 KDa
Source organism: Mycoplasma pneumoniae M129
Expression system: cell free system
UniProt:
  • Canonical: P75510 (Residues: 1-346; Coverage: 100%)
Gene names: MP568, MPN_265, trpS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand WSA 4 x WSA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P2
Unit cell:
a: 88.163Å b: 52.109Å c: 194.092Å
α: 90° β: 96.05° γ: 90°
R-values:
R R work R free
0.192 0.192 0.251
Expression system: cell free system

Quick links