› 2yya

X-ray diffraction
2.4Å resolution

Crystal structure of GAR synthetase from Aquifex aeolicus

Released:
DOI: 10.2210/pdb2yya/pdb
PDB entry 2yya coloured by chain, front view.
PDB entry 2yya coloured by chain, side view.
PDB entry 2yya coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structures of glycinamide ribonucleotide synthetase, PurD, from thermophilic eubacteria.
Sampei G, Baba S, Kanagawa M, Yanai H, Ishii T, Kawai H, Fukai Y, Ebihara A, Nakagawa N, Kawai G
J Biochem 148 429-38 (2010)
PMID: 20716513

Function and Biology Details

Reaction catalysed: 
ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylamine--glycine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 424 amino acids
Theoretical weight: 47.5 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66949 (Residues: 1-424; Coverage: 100%)
Gene names: aq_742, purD
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21
Unit cell:
a: 97.262Å b: 45.152Å c: 103.484Å
α: 90° β: 103.87° γ: 90°
R-values:
R R work R free
0.225 0.225 0.249
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Helicobacter pylori relies primarily on the purine salvage pathway for purine nucleotide biosynthesis.
Liechti et al. (2012)
8 more

2 mentions without citation

Structural studies of tri-functional human GART.
Welin et al. (2010)
1 more