› 2yzs

X-ray diffraction
2Å resolution

Crystal structure of uncharacterized conserved protein from Aquifex aeolicus

Released:
DOI: 10.2210/pdb2yzs/pdb
PDB entry 2yzs coloured by chain, front view.
PDB entry 2yzs coloured by chain, side view.
PDB entry 2yzs coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Ebihara A, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 36.9 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66692 (Residues: 2-316; Coverage: 100%)
Gene names: aq_369, cas1
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2
Unit cell:
a: 122.235Å b: 96.979Å c: 93.495Å
α: 90° β: 127.34° γ: 90°
R-values:
R R work R free
0.219 0.219 0.254
Expression system: Escherichia coli

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