› 2z02

X-ray diffraction
2.03Å resolution

Crystal structure of phosphoribosylaminoimidazolesuccinocarboxamide synthase wit ATP from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb2z02/pdb
PDB entry 2z02 coloured by chain, front view.
PDB entry 2z02 coloured by chain, side view.
PDB entry 2z02 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Kanagawa M, Baba S, Kuramitsu S, Yokoyama S, Kawai G, Sampei G, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 27.8 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58987 (Residues: 1-242; Coverage: 100%)
Gene names: MJ1592, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ATP 2 x ATP
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 82.099Å b: 82.099Å c: 85.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.217
Expression system: Escherichia coli

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