› 2z04

X-ray diffraction
2.35Å resolution

Crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit from Aquifex aeolicus

Released:
DOI: 10.2210/pdb2z04/pdb
PDB entry 2z04 coloured by chain, front view.
PDB entry 2z04 coloured by chain, side view.
PDB entry 2z04 coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Okada K, Tamura S, Baba S, Kanagawa M, Kawai G, Sampei G, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 42.29 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66608 (Residues: 1-365; Coverage: 100%)
Gene names: aq_245, purK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P1
Unit cell:
a: 51.291Å b: 55.053Å c: 74.541Å
α: 93.93° β: 101.54° γ: 114.23°
R-values:
R R work R free
0.232 0.232 0.267
Expression system: Escherichia coli

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