› 2z0y

X-ray diffraction
2.8Å resolution

Crystal structure of TTHA0657-SAM complex

Released:
DOI: 10.2210/pdb2z0y/pdb
PDB entry 2z0y coloured by chain, front view.
PDB entry 2z0y coloured by chain, side view.
PDB entry 2z0y coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Murayama K, Terada T, Kuramitsu S, Shirouzu M, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-178090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 24.8 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKI6 (Residues: 1-228; Coverage: 100%)
Gene name: TTHA0657
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P21
Unit cell:
a: 53.705Å b: 78.189Å c: 53.545Å
α: 90° β: 114.42° γ: 90°
R-values:
R R work R free
0.26 0.26 0.308
Expression system: Escherichia coli

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