› 2z1e

X-ray diffraction
1.55Å resolution

Crystal structure of HypE from Thermococcus kodakaraensis (outward form)

Released:
DOI: 10.2210/pdb2z1e/pdb
PDB entry 2z1e coloured by chain, front view.
PDB entry 2z1e coloured by chain, side view.
PDB entry 2z1e coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Crystal structures of [NiFe] hydrogenase maturation proteins HypC, HypD, and HypE: insights into cyanation reaction by thiol redox signaling.
Watanabe S, Matsumi R, Arai T, Atomi H, Imanaka T, Miki K
Mol Cell 27 29-40 (2007)
PMID: 17612488

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-177320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase expression/formation protein HypE Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 35.95 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JII7 (Residues: 1-338; Coverage: 100%)
Gene name: TK1993
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 88.345Å b: 45.833Å c: 75.052Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.204
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The ferredoxin/thioredoxin system of oxygenic photosynthesis.
Schürmann et al. (2008)
6 more

2 mentions without citation

Structural basis for the reaction mechanism of S-carbamoylation of HypE by HypF in the maturation of [NiFe]-hydrogenases.
Shomura et al. (2012)
1 more