› 2z1w

X-ray diffraction
1.63Å resolution

tRNA guanine transglycosylase TGT E235Q mutant in complex with BDI (2-BUTYL-5,6-DIHYDRO-1H-IMIDAZO[4,5-D]PYRIDAZINE-4,7-DIONE)

Released:
DOI: 10.2210/pdb2z1w/pdb
PDB entry 2z1w coloured by chain, front view.
PDB entry 2z1w coloured by chain, side view.
PDB entry 2z1w coloured by chain, top view.
Source organism: Zymomonas mobilis
Primary publication:
Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies.
Tidten N, Stengl B, Heine A, Garcia GA, Klebe G, Reuter K
J Mol Biol 374 764-76 (2007)
PMID: 17949745

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.92 KDa
Source organism: Zymomonas mobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand BDI 1 x BDI
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 90.878Å b: 65.324Å c: 70.615Å
α: 90° β: 96.65° γ: 90°
R-values:
R R work R free
0.134 not available 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Base ionization and ligand binding: how small ribozymes and riboswitches gain a foothold in a protein world.
Liberman et al. (2011)
5 other articles