› 2z21

X-ray diffraction
1.8Å resolution

Crystal Structure of a five site mutated Cyanovirin-N

Released:
DOI: 10.2210/pdb2z21/pdb
PDB entry 2z21 coloured by chain, front view.
PDB entry 2z21 coloured by chain, side view.
PDB entry 2z21 coloured by chain, top view.
Source organism: Nostoc ellipsosporum
Primary publication:
A monovalent mutant of cyanovirin-N provides insight into the role of multiple interactions with gp120 for antiviral activity.
Fromme R, Katiliene Z, Giomarelli B, Bogani F, Mc Mahon J, Mori T, Fromme P, Ghirlanda G
Biochemistry 46 9199-207 (2007)
PMID: 17636873

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanovirin-N Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 11.95 KDa
Source organism: Nostoc ellipsosporum
Expression system: Escherichia coli
UniProt:
  • Canonical: P81180 (Residues: 1-101; Coverage: 100%)
Sequence domains: CVNH domain
Structure domains: Cyanovirin-N

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 49.457Å b: 38.143Å c: 56.039Å
α: 90° β: 99.98° γ: 90°
R-values:
R R work R free
0.167 0.164 0.235
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cyanobacterial lectins characteristics and their role as antiviral agents.
Singh et al. (2017)
2 more

2 mentions without citation

Solution and crystal structures of a sugar binding site mutant of cyanovirin-N: no evidence of domain swapping.
Matei et al. (2008)
1 more