› 2z39

X-ray diffraction
1.7Å resolution

Crystal structure of Brassica juncea chitinase catalytic module Glu234Ala mutant (Bjchi3-E234A)

Released:
DOI: 10.2210/pdb2z39/pdb
PDB entry 2z39 coloured by chain, front view.
PDB entry 2z39 coloured by chain, side view.
PDB entry 2z39 coloured by chain, top view.
Source organism: Brassica juncea
Primary publication:
Crystal structures of a family 19 chitinase from Brassica juncea show flexibility of binding cleft loops.
Ubhayasekera W, Tang CM, Ho SWT, Berglund G, Bergfors T, Chye ML, Mowbray SL
FEBS J 274 3695-3703 (2007)
PMID: 17608716

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitin-binding type-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.2 KDa
Source organism: Brassica juncea
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9SQF7 (Residues: 146-389; Coverage: 64%)
Sequence domains: Chitinase class I
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 19 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 60.988Å b: 47.254Å c: 77.668Å
α: 90° β: 100.85° γ: 90°
R-values:
R R work R free
0.18 0.178 0.222
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Structural and functional evolution of chitinase-like proteins from plants.
Kesari et al. (2015)
1 more