› 2z42

X-ray diffraction
1.6Å resolution

Crystal Structure of Family 7 Alginate Lyase A1-II' from Sphingomonas sp. A1

Released:
DOI: 10.2210/pdb2z42/pdb
PDB entry 2z42 coloured by chain, front view.
PDB entry 2z42 coloured by chain, side view.
PDB entry 2z42 coloured by chain, top view.
Source organism: Sphingomonas sp.
Entry authors: Ogura K, Yamasaki M, Hashimoto W, Mikami B, Murata K

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 25.92 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WP3 (Residues: 81-308; Coverage: 74%)
Gene name: aly
Sequence domains: Alginate lyase
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 34.335Å b: 41.329Å c: 133.378Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.218
Expression system: Escherichia coli

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