› 2z4s

X-ray diffraction
3Å resolution

Crystal structure of domain III from the Thermotoga maritima replication initiation protein DnaA

Released:
DOI: 10.2210/pdb2z4s/pdb
PDB entry 2z4s coloured by chain, front view.
PDB entry 2z4s coloured by chain, side view.
PDB entry 2z4s coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
A common mechanism for the ATP-DnaA-dependent formation of open complexes at the replication origin.
Ozaki S, Kawakami H, Nakamura K, Fujikawa N, Kagawa W, Park SY, Yokoyama S, Kurumizaka H, Katayama T
J Biol Chem 283 8351-62 (2008)
PMID: 18216012

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosomal replication initiator protein DnaA Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 50.38 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: P46798 (Residues: 1-440; Coverage: 100%)
Gene names: TM_0926, dnaA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R32
Unit cell:
a: 221.817Å b: 221.817Å c: 55.602Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.291 0.233 0.291
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Regulating DNA replication in bacteria.
Skarstad et al. (2013)
26 more

6 mentions without citation

The DnaA Cycle in Escherichia coli: Activation, Function and Inactivation of the Initiator Protein.
Katayama et al. (2017)
5 more