› 2z4t

X-ray diffraction
2.5Å resolution

Crystal Structure of Vibrionaceae Photobacterium sp. JT-ISH-224 2,6-sialyltransferase in a Ternary Complex with Donor Product CMP and Accepter Substrate Lactose

Released:
DOI: 10.2210/pdb2z4t/pdb
PDB entry 2z4t coloured by chain, front view.
PDB entry 2z4t coloured by chain, side view.
PDB entry 2z4t coloured by chain, top view.
Source organism: Photobacterium sp. JT-ISH-224
Primary publication:
Crystal structure of Vibrionaceae Photobacterium sp. JT-ISH-224 alpha2,6-sialyltransferase in a ternary complex with donor product CMP and acceptor substrate lactose: catalytic mechanism and substrate recognition.
Kakuta Y, Okino N, Kajiwara H, Ichikawa M, Takakura Y, Ito M, Yamamoto T
Glycobiology 18 66-73 (2008)
PMID: 17962295

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-galactoside alpha-2,6-sialyltransferase Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 58.57 KDa
Source organism: Photobacterium sp. JT-ISH-224
Expression system: Escherichia coli
UniProt:
  • Canonical: A8QYL1 (Residues: 1-514; Coverage: 100%)
Gene name: pst6-224
Sequence domains: Sialyltransferase PMO188
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
4 bound ligands:
Ligand C5P 1 x C5P
Ligand GOL 5 x GOL
Ligand PO4 2 x PO4
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3121
Unit cell:
a: 90.292Å b: 90.292Å c: 204.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.243
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration.
Schnaar et al. (2014)
8 more

7 mentions without citation

Glycosyltransferase engineering for carbohydrate synthesis.
McArthur et al. (2016)
6 more