› 2z51

X-ray diffraction
1.35Å resolution

Crystal structure of Arabidopsis CnfU involved in iron-sulfur cluster biosynthesis

Released:
DOI: 10.2210/pdb2z51/pdb
PDB entry 2z51 coloured by chain, front view.
PDB entry 2z51 coloured by chain, side view.
PDB entry 2z51 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural analysis of Arabidopsis CnfU protein: an iron-sulfur cluster biosynthetic scaffold in chloroplasts.
Yabe T, Yamashita E, Kikuchi A, Morimoto K, Nakagawa A, Tsukihara T, Nakai M
J Mol Biol 381 160-73 (2008)
PMID: 18585737

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NifU-like protein 2, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 16.82 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93W20 (Residues: 83-235; Coverage: 65%)
Gene names: At5g49940, CNFU2, K9P8.16, NFU2, NIFU2
Sequence domains: NifU-like domain
Structure domains: Fe-S cluster assembly (FSCA)

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P222
Unit cell:
a: 43.546Å b: 57.394Å c: 61.303Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.135 0.173
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Ancient and essential: the assembly of iron-sulfur clusters in plants.
Balk et al. (2011)
2 more

2 mentions without citation

Structural/Functional Properties of Human NFU1, an Intermediate [4Fe-4S] Carrier in Human Mitochondrial Iron-Sulfur Cluster Biogenesis.
Cai et al. (2016)
1 more