› 2z5w

X-ray diffraction
1.35Å resolution

tBclA, a recombinant spore surface protein from Bacillus anthracis

Released:
DOI: 10.2210/pdb2z5w/pdb
PDB entry 2z5w coloured by chain, front view.
PDB entry 2z5w coloured by chain, side view.
PDB entry 2z5w coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Construction, crystal structure and application of a recombinant protein that lacks the collagen-like region of BclA from Bacillus anthracis spores.
Liu CQ, Nuttall SD, Tran H, Wilkins M, Streltsov VA, Alderton MR
Biotechnol Bioeng 99 774-82 (2008)
PMID: 17879302

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BclA C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 13.52 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WA8 (Residues: 236-370; Coverage: 37%)
Gene name: bclA
Sequence domains: BclA C-terminal domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P6322
Unit cell:
a: 68.144Å b: 68.144Å c: 163.178Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.191
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Exosporium Layer of Bacterial Spores: a Connection to the Environment and the Infected Host.
Stewart GC. (2015)
9 other articles

2 mentions without citation

Structure of the Chlamydia trachomatis immunodominant antigen Pgp3.
Galaleldeen et al. (2013)
1 more