› 2z6f

X-ray diffraction
1.9Å resolution

Crystal structure of NEAT domain from Staphylococcus aureus in complex with heme

Released:
DOI: 10.2210/pdb2z6f/pdb
PDB entry 2z6f coloured by chain, front view.
PDB entry 2z6f coloured by chain, side view.
PDB entry 2z6f coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
Structural basis for multimeric heme complexation through a specific protein-heme interaction: the case of the third neat domain of IsdH from Staphylococcus aureus.
Watanabe M, Tanaka Y, Suenaga A, Kuroda M, Yao M, Watanabe N, Arisaka F, Ohta T, Tanaka I, Tsumoto K
J Biol Chem 283 28649-59 (2008)
PMID: 18667422

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-regulated surface determinant protein H Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.85 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q931P4 (Residues: 539-664; Coverage: 15%)
Gene names: SAV1731, harA, isdH, sasI
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21212
Unit cell:
a: 49.217Å b: 75.686Å c: 39.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
6 more

11 mentions without citation

Iron Acquisition in Mycobacterium tuberculosis.
Chao et al. (2019)
10 more