› 2z6y

X-ray diffraction
2Å resolution

Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state

Released:
DOI: 10.2210/pdb2z6y/pdb
PDB entry 2z6y coloured by chain, front view.
PDB entry 2z6y coloured by chain, side view.
PDB entry 2z6y coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.
Mizuno H, Mal TK, Wälchli M, Kikuchi A, Fukano T, Ando R, Jeyakanthan J, Taka J, Shiro Y, Ikura M, Miyawaki A
Proc Natl Acad Sci U S A 105 9227-32 (2008)
PMID: 18574155

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.78 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 63.889Å b: 95.829Å c: 74.011Å
α: 90° β: 115.62° γ: 90°
R-values:
R R work R free
0.208 0.208 0.249
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.
Newman et al. (2011)
21 more

4 mentions without citation

Optogenetically controlled protein kinases for regulation of cellular signaling.
Leopold et al. (2018)
3 more