› 2z8z

X-ray diffraction
1.8Å resolution

Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase

Released:
DOI: 10.2210/pdb2z8z/pdb
PDB entry 2z8z coloured by chain, front view.
PDB entry 2z8z coloured by chain, side view.
PDB entry 2z8z coloured by chain, top view.
Source organism: Pseudomonas sp. MIS38
Primary publication:
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation.
Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S
FEBS Lett 581 5060-4 (2007)
PMID: 17923123

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chain: A
Molecule details ›
Chain: A
Length: 617 amino acids
Theoretical weight: 64.58 KDa
Source organism: Pseudomonas sp. MIS38
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RBY1 (Residues: 1-617; Coverage: 100%)
Sequence domains: RTX calcium-binding nonapeptide repeat (4 copies)
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CA 11 x CA
Ligand ZN 2 x ZN
Ligand PT 1 x PT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 49.879Å b: 84.404Å c: 87.012Å
α: 90° β: 96.5° γ: 90°
R-values:
R R work R free
0.175 0.174 0.205
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
3 more

2 mentions without citation

Identification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent protein.
Park et al. (2012)
1 more