› 2za9

X-ray diffraction
2.1Å resolution

Crystal Structure of Alginate lyase A1-II' N141C/N199C

Released:
DOI: 10.2210/pdb2za9/pdb
PDB entry 2za9 coloured by chain, front view.
PDB entry 2za9 coloured by chain, side view.
PDB entry 2za9 coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Entry authors: Ogura K, Yamasaki M, Mikami B, Hashimoto W, Murata K

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.77 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q75WP3 (Residues: 81-308; Coverage: 74%)
Gene name: aly
Sequence domains: Alginate lyase
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 35.459Å b: 70.612Å c: 90.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.261
Expression system: Escherichia coli BL21(DE3)

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