› 2zbe

X-ray diffraction
3.8Å resolution

Calcium pump crystal structure with bound BeF3 in the absence of calcium and TG

Released:
DOI: 10.2210/pdb2zbe/pdb
PDB entry 2zbe coloured by chain, front view.
PDB entry 2zbe coloured by chain, side view.
PDB entry 2zbe coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump.
Toyoshima C, Norimatsu Y, Iwasawa S, Tsuda T, Ogawa H
Proc Natl Acad Sci U S A 104 19831-6 (2007)
PMID: 18077416

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 995 amino acids
Theoretical weight: 109.63 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 1-993; Coverage: 99%)
Gene name: ATP2A1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand BEF 2 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 133.869Å b: 91.592Å c: 248.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.293 0.293 0.327

Quick links

Citations

17 review citations

Ion channels versus ion pumps: the principal difference, in principle.
Gadsby DC. (2009)
16 more

17 mentions without citation

Crystal structure of the sodium-potassium pump (Na+,K+-ATPase) with bound potassium and ouabain.
Ogawa et al. (2009)
16 more