› 2zbt

X-ray diffraction
1.65Å resolution

Crystal structure of pyridoxine biosynthesis protein from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2zbt/pdb
PDB entry 2zbt coloured by chain, front view.
PDB entry 2zbt coloured by chain, side view.
PDB entry 2zbt coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Manzoku M, Ebihara A, Fujimoto Y, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal 5'-phosphate synthase subunit PdxS Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 297 amino acids
Theoretical weight: 32.44 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKD9 (Residues: 1-293; Coverage: 100%)
Gene names: TTHA0704, pdxS
Sequence domains: SOR/SNZ family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: R3
Unit cell:
a: 180.97Å b: 180.97Å c: 100.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 0.21
Expression system: Escherichia coli BL21(DE3)

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