› 2zbw

X-ray diffraction
2.1Å resolution

Crystal structure of thioredoxin reductase-like protein from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2zbw/pdb
PDB entry 2zbw coloured by chain, front view.
PDB entry 2zbw coloured by chain, side view.
PDB entry 2zbw coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Ebihara A, Manzoku M, Fujimoto Y, Kitamura Y, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin--NADP reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 36.44 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SL28 (Residues: 1-335; Coverage: 100%)
Gene name: TTHA0465
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P43212
Unit cell:
a: 61.364Å b: 61.364Å c: 371.197Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.259
Expression system: Escherichia coli

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