› 2zet

X-ray diffraction
3Å resolution

Crystal structure of the small GTPase Rab27B complexed with the Slp homology domain of Slac2-a/melanophilin

Released:
DOI: 10.2210/pdb2zet/pdb
PDB entry 2zet coloured by chain, front view.
PDB entry 2zet coloured by chain, side view.
PDB entry 2zet coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases.
Kukimoto-Niino M, Sakamoto A, Kanno E, Hanawa-Suetsugu K, Terada T, Shirouzu M, Fukuda M, Yokoyama S
Structure 16 1478-90 (2008)
PMID: 18940604

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187558 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-27B Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q99P58 (Residues: 1-201; Coverage: 92%)
Gene name: Rab27b
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Melanophilin Chains: C, D
Molecule details ›
Chains: C, D
Length: 153 amino acids
Theoretical weight: 17.72 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q91V27 (Residues: 1-146; Coverage: 25%)
Gene names: Ln, Mlph, Slac2a
Sequence domains: FYVE-type zinc finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
Ligand ZN 4 x ZN
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P41212
Unit cell:
a: 82.208Å b: 82.208Å c: 325.484Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.296
Expression system: Not provided

Quick links

Citations

11 review citations

Rab GEFs and GAPs.
Barr et al. (2010)
10 more

6 mentions without citation

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
5 more