› 2zfg

X-ray diffraction
1.59Å resolution

Structure of OMPF PORIN

Released:
DOI: 10.2210/pdb2zfg/pdb
PDB entry 2zfg coloured by chain, front view.
PDB entry 2zfg coloured by chain, side view.
PDB entry 2zfg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the OmpF porin: function in a colicin translocon.
Yamashita E, Zhalnina MV, Zakharov SD, Sharma O, Cramer WA
EMBO J 27 2171-80 (2008)
PMID: 18636093

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand C8E 2 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P321
Unit cell:
a: 116.923Å b: 116.923Å c: 51.346Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.218
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Filming biomolecular processes by high-speed atomic force microscopy.
Ando et al. (2014)
16 more

28 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
27 more