› 2zfu

X-ray diffraction
2Å resolution

Structure of the methyltransferase-like domain of nucleomethylin

Released:
DOI: 10.2210/pdb2zfu/pdb
PDB entry 2zfu coloured by chain, front view.
PDB entry 2zfu coloured by chain, side view.
PDB entry 2zfu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Epigenetic control of rDNA loci in response to intracellular energy status.
Murayama A, Ohmori K, Fujimura A, Minami H, Yasuzawa-Tanaka K, Kuroda T, Oie S, Daitoku H, Okuwaki M, Nagata K, Fukamizu A, Kimura K, Shimizu T, Yanagisawa J
Cell 133 627-39 (2008)
PMID: 18485871

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA-processing protein 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 24.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43159 (Residues: 242-456; Coverage: 47%)
Gene names: KIAA0409, NML, RRP8, hucep-1
Sequence domains: Hypothetical methyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P61
Unit cell:
a: 43.81Å b: 43.81Å c: 403.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

90 review citations

Reactive oxygen species, cellular redox systems, and apoptosis.
Circu et al. (2010)
89 more

3 mentions without citation

Molecular mechanism of S-adenosylmethionine sensing by SAMTOR in mTORC1 signaling.
Tang et al. (2022)
2 more