› 2zgi

X-ray diffraction
1.93Å resolution

Crystal Structure of Putative 4-amino-4-deoxychorismate lyase

Released:
DOI: 10.2210/pdb2zgi/pdb
PDB entry 2zgi coloured by chain, front view.
PDB entry 2zgi coloured by chain, side view.
PDB entry 2zgi coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure of putative 4-amino-4-deoxychorismate lyase from Thermus thermophilus HB8.
Padmanabhan B, Bessho Y, Ebihara A, Antonyuk SV, Ellis MJ, Strange RW, Kuramitsu S, Watanabe N, Hasnain SS, Yokoyama S
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 1234-9 (2009)
PMID: 20054118

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-amino-4-deoxychorismate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 27.34 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKM2 (Residues: 1-246; Coverage: 100%)
Gene name: TTHA0621
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand PLP 4 x PLP
Ligand PGE 2 x PGE
Ligand PEG 3 x PEG
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1, RIGAKU FR-D
Spacegroup: P212121
Unit cell:
a: 65.613Å b: 133.585Å c: 141.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.249
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives.
Tahara et al. (2021)
1 more

2 mentions without citation

Structure and catalytic mechanism of yeast 4-amino-4-deoxychorismate lyase.
Dai et al. (2013)
1 more